I have verified (blasted) the published Megahit assemblies by McKernan et al. (@Kevin_McKernan
) against the SV40 promoter sequence (317bp).
Here's respectively the longest matching contig:
- Pfizer 1: Identities = 245/245 (100%), Gaps = 0/245 (0%); 77.3% match
- Pfizer 2: Identities = 245/245 (100%), Gaps = 0/245 (0%); 77.3% match
- Moderna 1: Identities = Identities = 67/67 (100%), Gaps = 0/67 (0%); 21.1% match
- Moderna 2: Identities = 317/317 (100%), Gaps = 0/317 (0%); 100% match
While Megahit was not able to assemble the entire SV40 sequence with the Pfizer 1&2 and Moderna 1 samples, it found a 100% match against the Moderna 2 sample!
My conclusion, while I could not verify the original SARS2 sequence - in this case I agree with McKernan et al., and the SV40 promoter appears to be present in the COVID-19 vaccines.
Sources & Code:
- Samples from Study: https://osf.io/b9t7m/
- SV40: https://snapgene.com/plasmids/basic_cloning_vectors/SV40_promoter opened in SnapGene Viewer, saved as .fa file.
- Code for each sample:
#Covid #Covid19 #Pfizer #Moderna #DNA #mRNA
) against the SV40 promoter sequence (317bp).
Here's respectively the longest matching contig:
- Pfizer 1: Identities = 245/245 (100%), Gaps = 0/245 (0%); 77.3% match
- Pfizer 2: Identities = 245/245 (100%), Gaps = 0/245 (0%); 77.3% match
- Moderna 1: Identities = Identities = 67/67 (100%), Gaps = 0/67 (0%); 21.1% match
- Moderna 2: Identities = 317/317 (100%), Gaps = 0/317 (0%); 100% match
While Megahit was not able to assemble the entire SV40 sequence with the Pfizer 1&2 and Moderna 1 samples, it found a 100% match against the Moderna 2 sample!
My conclusion, while I could not verify the original SARS2 sequence - in this case I agree with McKernan et al., and the SV40 promoter appears to be present in the COVID-19 vaccines.
Sources & Code:
- Samples from Study: https://osf.io/b9t7m/
- SV40: https://snapgene.com/plasmids/basic_cloning_vectors/SV40_promoter opened in SnapGene Viewer, saved as .fa file.
- Code for each sample:
makeblastdb -in PbiV1-cs-Megahit-assem.fa -dbtype nucl
blastn -query sv40.fa -db PbiV1-cs-Megahit-assem.fa -evalue 1 -task megablast > Pfi1.crunch
#Covid #Covid19 #Pfizer #Moderna #DNA #mRNA
OSF
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