https://www.sphkinhibitor.com/index.php/2017/01/20/to-illustrate-the-impact-of-effectors-on-the-ccmr-interaction-the-curves-for-basal-binding-in-the-absence-effector-is-subtracted-from-the-curves-for-ccmr-binding-in-the-presence-of-the-tested-effec/