https://www.socialhelponline.com/2016/01/18/the-fifty-nine-primer-binds-at-the-start-of-the-third-ptb-area-of-hmtk1-residue-341-we-were-being-not-profitable-in-amplifying-a-cdna-making-use-of-primers-at-the-fifty-nine-finish-of-the-gene-desig/