http://www.beta-secretase.com/2016/01/18/the-columns-were-washed-sequentially-with-buffer-1-and-buffer-one-that-contains-25-mm-imidazole-bound-protein-was-eluted-with-buffer-1-made-up-of-500-mm-imidazole-and-loaded-on-to-16sixty-hiload-sup/