https://www.nf-kbinhibitor.com/index.php/2016/08/31/roughly-six-eight-hours-posttransfection-cells-ended-up-washed-with-pbs-stem-cell-systems-and-refreshing-full-media-was-extra-to-allow-for-manufacturing-of-the-reporter-for-an-extra-24-several-hour/