Mobile Phase in AFFINITY CHROMATOGRAPHY
–weaken and eliminate the analyte-ligand interaction

HPLC
one of the most powerful tools in

HPLC Similar to_________ chromatography but uses a liquid mobile phase

HPLC The technique is applicable to thermally fragile samples,

DETECTION METHODS FOR HPLC
1-UV – Ultraviolet light
2-RI – Refractive Index 3-MS – Mass Spectrometry Who is most popular

Ion exchange chromatography relies on the attraction between______ charged stationary phase

The ion exchanger consists of an inert support medium coupled covalently to positive (anion exchanger) or negative (cation exchanger) functional groups.

if anion exchange chromatography is performed, negatively charged sample components will interact more with the stationary phase

exchangers have positively charged with negatively charged mobile counter

Anion is

Cation is bacic

Tertiary amines is strong anion exchangers

Secondary amines is weak anion exchangers

Sulphonic acid is Strong cation exchangers carboxylic acid is Weak cation exchangers

Herbs is more expensive and perhaps more harsh

herbs do take longer to work and they aren’t as strong

Herbs can be an effective alternative, with fewer side effects

Used for depression, bulimia, OCD, Panic disorder

ST. JOHN’S WORT Used for depression, anxiety

SCULLCAP
Used for panic disorders, anxiety, nervousness