The factor governing separation of mixtures of solutes on filter paper is the partition between two miscible phases.
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water adsorbed on cellulose fibres in the paper (Mobile phase).

(TLC)
involves a stationary phase of a thin layer of adsorbent like

Detection under UV light is second choice - non destructive

Spots of fluorescent compounds can be seen at 254 nm (short wave length) or at 366 nm (long wave length)

Spots of non fluorescent compounds can be seen - fluorescent stationary phase is used - cellulose GF

GAS CHROMATOGRAPHY (GC)
Also called as Gas————- chromatography

stationary phase of GC (often a liquid silicone- based material

mobile gas of GC (most often nitrogen )

high temperatures used in GC make it suitable for high molecular weight biopolymers or proteins

mobile phase is an unreactive gas ( e.g. Nitrogen

stationary phase is an volatile liquid

Columns in GC are

GC DETECTORS

AFFINITY CHROMATOGRAPHY
Based on selective covalent interaction between an analyte and specific molecules

AFFINITY CHROMATOGRAPHY
Highly specific and selective

Mobile Phase in AFFINITY CHROMATOGRAPHY
–weaken and eliminate the analyte-ligand interaction

HPLC
one of the most powerful tools in

HPLC Similar to_________ chromatography but uses a liquid mobile phase

HPLC The technique is applicable to thermally fragile samples,

DETECTION METHODS FOR HPLC
1-UV – Ultraviolet light
2-RI – Refractive Index 3-MS – Mass Spectrometry Who is most popular