(Time for #PLANB - Zoom call, May 12th @ 4pm ET) Either rid the Centers for Disease Control of its capture by Big Pharma, or abolish it
with James Lyons-Weiler, Ph.D., Director, Institute for Pure and Applied Knowledge
Host: Charles Frohman
Wed, May 12th @ 4pm EST / 2pm MTN
Register here:
https://yourfreedomhub.com/CorruptCDC

Access the peer-reviewed publication explaining Plan B here:
https://bit.ly/3hjovHG
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This month's IPAK EDU Science Webinar will feature an in-depth analysis of COVID19 VAERS reports.
Note this month's TIME is 11AM EST Thursday, May 13th
https://ipak-edu.org/old-home-2/?store-page=IPAK-EDU-Monthly-Science-Webinar-p294327406
Please tag someone who needs to know.

PNAS #Sciencesez Reverse-transcribed SARS-CoV-2 RNA can integrate into the genome of cultured human cells and can be expressed in patient-derived tissues https://www.pnas.org/content/118/21/e2105968118 #COVID19 Once again, social media censors are proven wrong. Stand down and let science proceed.

My proposal for massive reform - a sober proposal for #PlanB - it's time to overhaul #publichealth from the ground up. Thank you Jeff Kanter and Charles Frohman - https://www.youtube.com/watch?v=ONEsRczjrn8

IPAK's James Lyons-Weiler presents an in-depth critique of public health in the US - Grand Rapids, Michigan, 5/5/2021 - https://gorf.tube/videos/watch/978989ec-b72c-4612-acad-91ea0013d9c7

CDC’S NEW UNMASKING RECOMMENDATIONS BASED ON BIASED STUDIES - jameslyonsweiler.com
https://jameslyonsweiler.com/2021/05/15/cdcs-new-unmasking-recommendations-based-on-biased-studies/

Peer-reviewed study
A Report on the US Vaccine Adverse Events Reporting System (VAERS) of the COVID-19 Messenger Ribonucleic Acid (mRNA) Biologicals - Jessica Rose, PhD -
https://www.publichealthpolicyjournal.com/clinical-and-translational-research

Support IPAK's latest research scientist, Jessica Rose, via this page.
https://ipaknowledge.org/joshua-kuntz-research-fellowship.php

LOADED EPISODE!!! My co-author, Dr. Paul Thomas, interviews Dr. Wakefield on the persecution of ethical physicians, Jeremy Hammond on the issue of censorship, Dr. Peter McCollough on COVID19 tx, and Bernadette Pajer discusses studies on natural vs. vaccine immunity. Also listen to what it's like to live with a life of seizures. Fight censorship by liking and following the accounts that share bona fide objective scientific research studies.

https://rumble.com/vh7rgl-against-the-wind-with-dr.-paul-episode-8.html

New peer-reviewed study shows serious problems with #VAERS safety assessment of HPV vaccine associated outcomes. #HPVVaccine. #Science
https://bit.ly/3fjtjvm

Course development is an ongoing effort @ IPAK-EDU - and we're excited to announce that Robert F. Kennedy, Jr. will giving an inaugural lecture "Environmental Messages for the Ages" exclusively as part of our newly minted course, "Environmental Toxicity: Ecosystem and Human Health", to be taught by Dr. James Lyons-Weiler, which starts in the Fall (Sept.). Learn the fundamental so you're ready for the fight! Between now and then we're offering both Biology A (learn the fundamentals!) and Bioinformatics (analyze some sequences)! For more information on these course, visit http://ipak-edu.org/

I'll be in Harrisburg, PA for this IPAK COVID-19 Science Townhall - https://ipak-edu.org/old-home-2/?store-page=IPAK-COVID-19-Science-Townhall-Harrisburg-PA-June-13-p360521535
IPAK COVID-19 Science Townhall - JUNE 13, 2021
Join Dr. Lyons-Weiler for a live educational workshop on COVID-19 facts and realities - what's been done that's been useful, what's been harmful - and what the future of public health will look like. Review of diagnosis paradigms, including sequence-based diagnosis, evidence for/against masking, treatments and what the published data say about the effects of COVID-19 vaccination. Get the latest on pathogenic priming and steps necessary to minimize exposure to immunotoxic viral epitopes.
LOCATION: You will be notified before the event

TIME: 6:30 PM

Sequence-based COVID19 testing is here. NAATEC consortium member Dr. Sin Hang Lee's lab has won CLIA certification to use Sanger sequencing to detect COVID19 in a more accurate manner. No more Ct threshold baloney. Dr. Lee show other lab how this is done. https://www.wtnh.com/news/health/coronavirus/new-type-of-covid-test-developed-in-milford-lab-samples-virus-infected-cells-for-more-accuracy/ #Science

Registration is still open - first bioinformatics class is today @ 1PM ET. Get some bioinformatics skills from an expert. Here's the syllabus. Register here - > https://ipak-edu.org/old-home-2/

SYLLABUS (Subject to change)

Biology 202

Bioinformatics

with Dr. Lyons-Weiler

Classes Start June 1, 2021

Amaze and astound your friends and family! Imagine if you could analyze any DNA, RNA or protein sequence and run analyses on it to determine its likely identity, function, structure, relationships to other nucleotide sequence. If it's an RNA sequence, what is its likely structure? If it's DNA, does it encode a protein? Does the protein have a likely special function, or cellular location? Does the protein have conserved domains? If it’s a viral or bacterial protein, does it have any immunogenic epitopes? If so, what are they, and do they match human proteins in a manner that might lead to pathogenic priming? Plus much, much more.

Course Description: This course will provide students with an introduction to some of the important fundamental skill sets in Bioinformatics. In-depth description of methods and algorithms will provide background and hands-on experience with software will provide practical experience. Concepts and approaches to DNA and amino acid sequence alignment, homology, conserved domain identification, phylogenetic inference, array-based transcriptomics, quantitative PCR analysis and peptide identification searches will be presented. Basic computing skills are required.

Learning Outcomes:

Students will

· Understand molecular biology of genes, transcription and translation beyond central dogma

· Search biological databases containing sequences, clinical, and structural information

· Retrieve information from these biological databases

· Perform structure/function analysis of biological molecules

· Perform DNA/protein sequence alignment and analysis

· Identify homologs and conserved domains

· Perform a basic phylogenetic inference from a multiple sequence alignment

· Identify differentially expressed genes in a microarray data set

· Be able to interpret the output of a quantitative RT-PCR experiment

· Understand peptide identification search algorithms given proteomics profiles

· Be able to detect structure in small RNAs

Time Tuesdays 1:00PM-3:00PM (usually lasts an hour or so)

Instructor: James Lyons-Weiler

Required Materials: Students will make extensive use of computers and the internet. PC software to be used includes various web applications.

Recommended Text(s): Recommended readings will be sent each week. The book that most closely parallels our in-class experiences is David Mount’s Bioinformatics: Sequence and Genome Analysis. However, every student of Biotechnology should get and read a copy of Campbell & Heyer’s Discovering Genomics, Proteomics and Bioinformatics.

Directed Study: Data analysis using web-based database searches, sequence analysis, protein structure analysis, phylogenetic trees, transcriptomics, proteomic peptide identification, qPCR. Interpretation of current journal articles involving bioinformatics and QA/QC.

Teaching Methods:

Emphasis on learning by doing – directed exercises in 1) use of a variety of web applications, 2) interpretation of results from those applications, 3) in-class/take-home exercises that build on past experiences, 4) in-class quizzes and exams.

The assignments and content will rely on web-based tutorials and specific assignments from bioinformatics websites. The tools and resources for bioinformatics are generally free to the public. They lend themselves to exploration, so students are encouraged to ask and seek answers for impromptu questions.

Course Plan:

1 Beyond the Central Dogma Course Overview.

Lecture on transcription, translation, alternative splicing, RNA editing, post-translational modification. Find splice variants (isoforms) of sequences with the same gene name in Genbank. Homework Assignment #1

2 Overview of Bioinformatics Resources.

General description of bioinformatics resources, introduction to NCBI, Genbank, Pubmed, Nucleotide, Protein Identify a gene of interest related to a disease or disease state of interest to the student. Find the nucleotide sequence in the Nucleotide database. Find the protein sequence in the Nucleotide entry and in the Protein database. Homework Assignment #2

3 BLAST Searching sequence databases.

Performing BLAST comparisons between two sequences. BLASTing one sequence against a chosen database In-class/take-home exercise. BLAST a sequence of interest against Nucleotide database @ NCBI and BLAST 2 Sequences. Homework Assignment #3

4 Pairwise Sequence Alignment

Principles of sequence alignment algorithm In-class/take-home exercise with alignment of two sequences. Homework Assignment #4

5 Studying Your Sequence Open Reading Frames and the Conserved Domain Database In-class/take-home exercise of finding open reading frames in nucleotide sequences using ORF, and finding conserved domains in protein sequences via the CDD. Homework Assignment #5

6 Identifying Immunogenic Epitopes in Pathogen Proteins

7 Identifying Potentially Autoreactogenic Epitopes in Pathogen Proteins

8 Conserved Domains Conserved Domain Database part 2 and Homologene

In-class/take-home exercise on identification of conserved domains in amino acid coding sequences and finding homologs. Homework Assignment #6

9 Phylogenetic Inference ClustalW multiple sequence alignment tools.

Tools for estimating phylogenetic trees. Derive a multiple alignment and a phylogenetic tree for homologs of the student’s sequence of interest.

Homework Assignment #7

10 Transcriptomics with Microarrays 1 The Basics Read an overview on transcriptomics with microarrays. None.

11 Transcriptomics with Microarrays 2

Normalization/Transformation; Identifying Differentially Expressed Genes Implement a simple analysis comparing gene expression in two clinical groups using a web application. Homework Assignment #8

12 Quantitative

PCR Interpretation of qRT-PCR results Given qRT-PCR output, perform interpretation of CT values. Homework Assignment #9

13 Proteomics

Peptide identification Mass-spec profile peptide identification/Mascot/Sequest/X-Tandem/Consensus Perform an online peptide fingerprint search Homework Assignment #10

14 Small RNAs

Computational Analysis of Small RNAs Use web tools to predict small RNA structure/function Homework Assignment #11

15 Summing it All Up

COVID-19 Diagnosis Numbers and Deaths Fiasco Continues

Dr. Jack and Dr. Henry Ealy get into the question of whether CDC changed their criterion for considering COVID-19 cases in the vaccinated compared to those in use since April 2020 - and they review the startling statistics on deaths and vaccine adverse events being reported following COVID-19 vaccination. Fact-checkers beware: We're fact-checking you AGAIN.

(IPAK CEO & Director Accuses CDC of Fraud) FACT-CHECKING THE FACT-CHECKERS: In a live #UNBREAKINGSCIENCE podcast today with guest Dr. Henry Ealy, IPAK CEO & Director Dr. James Lyons-Weiler, upon reviewing the facts of whether CDC changed the criteria for surveilling and reporting COVID-19 cases in persons vaccinated against COVID-19, realized that the CDC had changed the outcome measure during a post-market "pharmacovigilence" COVID-19 vaccine safety & efficacy trials. Dr. Lyons-Weiler (aka Dr. Jack) called the change "scientific fraud". Dr. Ealy and Dr. Jack debunk so-called "fact-checker" claims that CDC did not change the criteria for tracking COVID-19 cases among the vaccinated, and review recent data on deaths reported in the VAERS database. Dr. Ealy reported an anomaly where the number of deaths following the COVID-19 vaccine DECREASED this week compared to last.

Don't miss this episode of #UnbreakingScience - and please heed Dr. Jack's call to get this information to your Congressional Representatives and Senators. The video is currently available on YouTube - we'll see for how long!

https://www.youtube.com/watch?v=Rn5B9HBx3io
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