水水的摸鱼日常
@changeless
2.3K subscribers
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20 videos
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自用mark
频道所有转发完全不代表本人观点

菜得一逼的鶸鸡生物搬砖狗的搬砖日常
博士四年级在读 quit倒计时ing
除了生物什么都做

做过Archaea，UAA，CRISPR-Cas，evolution，microbiome，barcode，RNA regulation，imaging，dev bio

Paper/Seminar Record: https://t.me/PaperReading
My daily crap: https://t.me/loveuchangeless
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水水的摸鱼日常
2.3K subscribers
水水的摸鱼日常
https://www.synthego.com/guide/how-to-use-crispr/transfection-protocols
Synthego
CRISPR Transfection Protocols Guide: How To Select The Best Method
A key step of any CRISPR workflow is transfecting the gRNA and Cas9 into the target cells, but different transfection protocols have different advantages. This comprehensive guide covers all of the different transfection methods and discusses the best method…
135 views
水水的摸鱼日常
95 views
水水的摸鱼日常
水水的摸鱼日常
https://www.synthego.com/guide/how-to-use-crispr/transfection-protocols
https://www.synthego.com/guide/how-to-use-crispr
Synthego
How To Use CRISPR: Your Guide to Successful Genome Engineering
The CRISPR-Cas9 system has revolutionized the field of genome engineering with limitless applications in disease therapeutics, drug discovery, agriculture, biofuels, and much more. This comprehensive guide addresses all the main steps in the CRISPR workflow…
124 views
水水的摸鱼日常
https://www.thermofisher.com/us/en/home/life-science/genome-editing/geneart-crispr/crispr-libraries/trueguide-grnas.html
Thermofisher
TrueGuide Synthetic gRNA | Thermo Fisher Scientific - UK
Find the right synthetic guide RNA using our TrueGuide Synthetic guide RNA search tool. Download the TrueGuide Synthetic gRNA user guide and explore validated protocols.
109 views
水水的摸鱼日常
https://international.neb.com/protocols/2016/03/17/t7-endonuclease-i-mutation-detection-with-the-engen-mutation-detection-kit-e3321
Neb
T7 Endonuclease I-based Mutation Detection with the EnGen® Mutation Detection Kit (NEB #E3321) | NEB
New users are encouraged to perform PCR and T7 Endonuclease I digestion using the included control template and primer mix
125 views
水水的摸鱼日常
今日感想：你校基础科学不行那必然是有原因的
122 views
水水的摸鱼日常
lab的外国人半夜来发现autoclave坏了 专门搜了两个中国字写来发给师姐哈哈哈哈哈哈哈哈
467 views
水水的摸鱼日常
Lab有一个阿姨辈的移民technician在lab放了空气炸锅高压锅等等…每周末都等着被投喂（我
今天的新奥尔良烤翅太好吃了！吃了一盘！
383 views
水水的摸鱼日常
197 views
水水的摸鱼日常
开会博后师姐对博士师兄发火，连着老板一起凶。博士开完会立刻买了一只章鱼来送给师姐。告诉她正常时候是微笑章鱼，但是以后想凶老板就翻到蓝色的凶凶的背面给老板看！
1.76K views
水水的摸鱼日常
https://zhuanlan.zhihu.com/p/346821293?utm_source=wechat_timeline&utm_medium=social&utm_oi=1200243600540680192&s_r=0
241 views
水水的摸鱼日常
草
今天的Talker是alexander van oudenaarden
他paper出现在The Big Bang Theory里面 因为Penny tried to pronounce his paper name to inpress her boyfriend —> Magneto-electric Aharonov-Bohm effect in metal rings
191 views
水水的摸鱼日常
166 views
水水的摸鱼日常
现在这个傻逼政策就是：
我们三个人都在实验室
但如果我们要开会讨论
我们要分别去三个不同的没有人的房间然后Zoom
……
181 views
水水的摸鱼日常
现在的我：
我事情好多做不完了……难道老板真的写完了今晚due的grant？不应该赶着写grant取消今天的meeting吗！可是我还没有收到取消的邮件QAQ
136 views
水水的摸鱼日常
水水的摸鱼日常
现在的我： 我事情好多做不完了……难道老板真的写完了今晚due的grant？不应该赶着写grant取消今天的meeting吗！可是我还没有收到取消的邮件QAQ
Update: 取消了（要是早点说我就可以把我五点的会议改到现在了就不用开到晚上去了嘤嘤嘤
125 views
水水的摸鱼日常
隔壁学校的年轻华人老板 老婆在Facebook上班 xmsl
117 views
水水的摸鱼日常
xmsl
羡慕engineering，羡慕ML
华科的ME
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水水的摸鱼日常
老板明明说是给我发 却没有发我的邮箱 ❓
173 views
水水的摸鱼日常
草
184 views