水水的摸鱼日常
@changeless
2.3K subscribers
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20 videos
49 files
2.08K links
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频道所有转发完全不代表本人观点

菜得一逼的鶸鸡生物搬砖狗的搬砖日常
博士四年级在读 quit倒计时ing
除了生物什么都做

做过Archaea，UAA，CRISPR-Cas，evolution，microbiome，barcode，RNA regulation，imaging，dev bio

Paper/Seminar Record: https://t.me/PaperReading
My daily crap: https://t.me/loveuchangeless
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水水的摸鱼日常
2.3K subscribers
水水的摸鱼日常
https://awardsdatabase.usc.edu/app/index.asp
84 views
水水的摸鱼日常
啊 生活
84 views
水水的摸鱼日常
Forwarded from 每个女孩都是最美的女孩 (Sakana @返工了)
草 现在的庆祝是不是有点太假了
73 views
水水的摸鱼日常
https://zhuanlan.zhihu.com/p/64336174
知乎专栏
怎么养细胞？看这一篇就够了！
养细胞这件事儿，看似简单，然而却常常因为各种原因，让我们珍贵的实验细胞一再受损或者死亡，今天学霸姐姐就结合多年实践经验和细胞培养指南，为大家讲述细胞从复苏、传代到冻存等一系列过程及常遇到的那些你可能…
83 views
水水的摸鱼日常
https://zhuanlan.zhihu.com/p/59667740
知乎专栏
细胞培养必备常识，你掌握了吗？
细胞培养，实验课题咨询可私信。用专业的心、做专业的事。加速您的科研进程！ 1.如何选择培养基？培养某一类型细胞没有固定的培养条件。在 MEM 中培养的细胞，很可能在 DMEM 或 M199 中同样很容易生长。总之，首选…
195 views
水水的摸鱼日常
https://2019.igem.org/wiki/images/3/31/T--NUDT_CHINA--Protocol_for_Cell_Culture_and_Subculture.pdf
94 views
水水的摸鱼日常
https://horizondiscovery.com/-/media/Files/Horizon/resources/Technical-manuals/tla-hek293t-cell-line-manual.pdf
91 views
水水的摸鱼日常
https://www.thermofisher.com/us/en/home/references/gibco-cell-culture-basics/cell-culture-protocols/freezing-cells.html
Thermofisher
Cell Freezing Protocols | Thermo Fisher Scientific - UK
Learn how to freeze cells using this step-by-step cell freezing protocol. Get a list of necessary materials and tips for freezing cells.
103 views
水水的摸鱼日常
https://www.synthego.com/guide/how-to-use-crispr/transfection-protocols
Synthego
CRISPR Transfection Protocols Guide: How To Select The Best Method
A key step of any CRISPR workflow is transfecting the gRNA and Cas9 into the target cells, but different transfection protocols have different advantages. This comprehensive guide covers all of the different transfection methods and discusses the best method…
135 views
水水的摸鱼日常
95 views
水水的摸鱼日常
水水的摸鱼日常
https://www.synthego.com/guide/how-to-use-crispr/transfection-protocols
https://www.synthego.com/guide/how-to-use-crispr
Synthego
How To Use CRISPR: Your Guide to Successful Genome Engineering
The CRISPR-Cas9 system has revolutionized the field of genome engineering with limitless applications in disease therapeutics, drug discovery, agriculture, biofuels, and much more. This comprehensive guide addresses all the main steps in the CRISPR workflow…
124 views
水水的摸鱼日常
https://www.thermofisher.com/us/en/home/life-science/genome-editing/geneart-crispr/crispr-libraries/trueguide-grnas.html
Thermofisher
TrueGuide Synthetic gRNA | Thermo Fisher Scientific - UK
Find the right synthetic guide RNA using our TrueGuide Synthetic guide RNA search tool. Download the TrueGuide Synthetic gRNA user guide and explore validated protocols.
109 views
水水的摸鱼日常
https://international.neb.com/protocols/2016/03/17/t7-endonuclease-i-mutation-detection-with-the-engen-mutation-detection-kit-e3321
Neb
T7 Endonuclease I-based Mutation Detection with the EnGen® Mutation Detection Kit (NEB #E3321) | NEB
New users are encouraged to perform PCR and T7 Endonuclease I digestion using the included control template and primer mix
125 views
水水的摸鱼日常
今日感想：你校基础科学不行那必然是有原因的
122 views
水水的摸鱼日常
lab的外国人半夜来发现autoclave坏了 专门搜了两个中国字写来发给师姐哈哈哈哈哈哈哈哈
467 views
水水的摸鱼日常
Lab有一个阿姨辈的移民technician在lab放了空气炸锅高压锅等等…每周末都等着被投喂（我
今天的新奥尔良烤翅太好吃了！吃了一盘！
383 views
水水的摸鱼日常
197 views
水水的摸鱼日常
开会博后师姐对博士师兄发火，连着老板一起凶。博士开完会立刻买了一只章鱼来送给师姐。告诉她正常时候是微笑章鱼，但是以后想凶老板就翻到蓝色的凶凶的背面给老板看！
1.76K views
水水的摸鱼日常
https://zhuanlan.zhihu.com/p/346821293?utm_source=wechat_timeline&utm_medium=social&utm_oi=1200243600540680192&s_r=0
241 views
水水的摸鱼日常
草
今天的Talker是alexander van oudenaarden
他paper出现在The Big Bang Theory里面 因为Penny tried to pronounce his paper name to inpress her boyfriend —> Magneto-electric Aharonov-Bohm effect in metal rings
191 views