EB1-GFP was purified as in ref. 31 and used at a final concentration of 60 nM. For dynein inhibition, the p150-CC1 fragment of dynactin was purified as in ref. 17 and used at a final concentration of 0.04 mg/mL
https://www.pnas.org/doi/full/10.1073/pnas.1418796111
https://www.pnas.org/doi/full/10.1073/pnas.1418796111
PNAS
Microtubule nucleation remote from centrosomes may explain how asters span large cells | PNAS
A major challenge in cell biology is to understand how nanometer-sized molecules can
organize micrometer-sized cells in space and time. One solutio...
organize micrometer-sized cells in space and time. One solutio...
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