老板让我去找别的lab借仪器
我：you know. If I need to talk to people, I have to spend at least 1 h to be mentally prepared.
老板：…
模拟老板OS：你跟我吵架的时候没见你这么社恐
我：you know. If I need to talk to people, I have to spend at least 1 h to be mentally prepared.
老板：…
模拟老板OS：你跟我吵架的时候没见你这么社恐
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美国人不要太过分。我们排队买冰淇淋，前面一对美国夫妇，买了两个冰淇淋，各俩球，一共五欧，给了十欧，转身就走。意大利老阿姨拿着找钱追出去。他回过头潇洒的说，keep the change，我们来自美国。啥意思，散财童子吗。老阿姨站在那里朗声大笑，说，BRAVO～ source
给undergrad说 我们下学期有new grad students，一到两个
undergrads的第一反应：are they asians?
你lab的asians魔咒已经走不脱了，中日韩全方位进击
undergrads的第一反应：are they asians?
你lab的asians魔咒已经走不脱了，中日韩全方位进击
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Protein Expression and Purification
CC1 was expressed from pVEX-CC1 (Quintyne et al., 1999) in BL21(DE3) cells and purified as described previously (de Cuevas et al., 1992) with minor modifications. Briefly, after overnight protein expression, cells were resuspended in Bugbuster reagent (Novagen, Madison, WI) with 25 U/ml benzonase DNase (Novagen), rocked at room temperature for 20 min, and spun at 16,000 × g for 20 min at 4°C. The supernatant was fractionated by 25% ammonium sulfate precipitation, the pellet was resuspended in phosphate buffer (16 mM Na2HPO4, 4 mM NaH2PO4, 2 mM dithiothreitol, 1 mM EDTA, 0.1 mM phenylmethylsulfonyl fluoride, pH 7.2), brought to 0.75 M KCl, and boiled in a water bath for 3 min. After clarification by centrifugation, the supernatant was loaded onto a MonoQ HR 5/5 anion exchange column (Amersham Biosciences, Piscataway, NJ) at 1 ml/min. The protein was eluted using a 0-1000 mM KCl gradient in 20 mM Tris-Cl, pH 7.0. The protein was concentrated using an Amicon centricon (3000 MW cut-off; Millipore, Bedford, MA). CC2 was expressed from pVEX-CC2 (Quintyne et al., 1999) in BL21(DE3)pLysS cells and purified as described above, except that a 50% ammonium sulfate precipitation was used.
https://www.molbiolcell.org/doi/10.1091/mbc.e03-01-0025?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%20%200pubmed
CC1 was expressed from pVEX-CC1 (Quintyne et al., 1999) in BL21(DE3) cells and purified as described previously (de Cuevas et al., 1992) with minor modifications. Briefly, after overnight protein expression, cells were resuspended in Bugbuster reagent (Novagen, Madison, WI) with 25 U/ml benzonase DNase (Novagen), rocked at room temperature for 20 min, and spun at 16,000 × g for 20 min at 4°C. The supernatant was fractionated by 25% ammonium sulfate precipitation, the pellet was resuspended in phosphate buffer (16 mM Na2HPO4, 4 mM NaH2PO4, 2 mM dithiothreitol, 1 mM EDTA, 0.1 mM phenylmethylsulfonyl fluoride, pH 7.2), brought to 0.75 M KCl, and boiled in a water bath for 3 min. After clarification by centrifugation, the supernatant was loaded onto a MonoQ HR 5/5 anion exchange column (Amersham Biosciences, Piscataway, NJ) at 1 ml/min. The protein was eluted using a 0-1000 mM KCl gradient in 20 mM Tris-Cl, pH 7.0. The protein was concentrated using an Amicon centricon (3000 MW cut-off; Millipore, Bedford, MA). CC2 was expressed from pVEX-CC2 (Quintyne et al., 1999) in BL21(DE3)pLysS cells and purified as described above, except that a 50% ammonium sulfate precipitation was used.
https://www.molbiolcell.org/doi/10.1091/mbc.e03-01-0025?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%20%200pubmed
Molecular Biology of the Cell
Analysis of the Dynein-Dynactin Interaction In Vitro and In Vivo | Molecular Biology of the Cell
Cytoplasmic dynein and dynactin are megadalton-sized multisubunit molecules that function
together as a cytoskeletal motor. In the present study, we explore the mechanism of
dynein-dynactin binding in vitro and then extend our findings to an in vivo context.…
together as a cytoskeletal motor. In the present study, we explore the mechanism of
dynein-dynactin binding in vitro and then extend our findings to an in vivo context.…
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美国的大学教授分几级？简单的回答是不分级，只有职称高低之分，正教授里面，又有讲席教授(有常年固定研究经费）与普通教授之别，但也谈不上不同级别。加州大学也不分教授级别，但教授的工资有级别，例如正教授的工资一共九级，最高的是第九级，去年的标准为188400美元。有时校方为了留人，会向被挖的教授提供高于第九级的年薪，也就是”above scale”，即超级的。一旦超级，就没有循序渐进的标准了，可以是20万，也可以是30万，要看挖人的学校提供的价码。总之，美国大学教授的工资高度市场化，能折腾、不断跳槽或跳槽可能性极高的教授，工资节节上升，会远远高于甚至数倍于同领域其他教授。就文科而言，基本上出书越多，影响力越大，被挖的机会越高，年薪也涨得越快。相反，不做学问的、不善折腾的，工资如蜗牛爬行，差距会越拉越大。 source
Forwarded from 每个女孩都是最美的女孩
周五的下午
做实验的间隙，起因是一个本科生（pre-med)在犹豫自己要不要考mcat bc she can’t see herself as a doctor in the future, 相反她更想要自己的business，然后本科生，本科生，tech，我，可以聚在一起聊人生聊未来聊迷茫聊经历
真美好啊 是这个年龄独有的迷茫但永远探索永远尝试的年龄
然后我们开始说老板坏话（不是x），然后越说越high了，结果老板开门进来，四个人突然安静然后爆笑出来
老板：？
做实验的间隙，起因是一个本科生（pre-med)在犹豫自己要不要考mcat bc she can’t see herself as a doctor in the future, 相反她更想要自己的business，然后本科生，本科生，tech，我，可以聚在一起聊人生聊未来聊迷茫聊经历
真美好啊 是这个年龄独有的迷茫但永远探索永远尝试的年龄
然后我们开始说老板坏话（不是x），然后越说越high了，结果老板开门进来，四个人突然安静然后爆笑出来
老板：？
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Forwarded from Web to Album
今天再次分享一下我认识的人里的人类天花板：
之前我有提过好多次这个天才女孩：她12岁进入UCLA读本科（数学专业），14岁本科毕业，顺利考入Caltech（加州理工学院，关于这个学校有多牛逼我相信懂得都懂）读博，数学专业，并于20岁毕业，成为当时该校有史以来最年轻的女博士（也是学数学）。
我有幸和她坐在同一间教室听课，也是因为她获得博士学位后，心血来潮回母校看看老师。当时坐在大神后面每天都很激动，我一直称她为偶像
后来我就再也没有看到她的任何信息了。刚和虎虎提到她的时候，心血来潮上网一查，发现她在获得数学博士学位后，一直在做关于核磁共振的研究，没想到她竟然在医学领域越走越远，直接考入了UCLA医学院（可以说是顶尖医学院之一）读医去了，现在已经是一名医生了！喜提MD PhD双title！
太太太太牛了！感觉我就像是读了一本爽文一样此时此刻好激动好感动，谁懂🙉！
（图3是她当时回我的email，我当时下课时问了她一个我想不明白的微积分相关的知识（现在已经完全不记得了），她想了想然后说回家email给我，我当时以为只是客套话，结果没想到两天后收到她很认真的讲解，瞬间解开了我的疑惑，我回送了颗巧克力答谢。真就是那种”another story to tell your grandchildren”） source
之前我有提过好多次这个天才女孩：她12岁进入UCLA读本科（数学专业），14岁本科毕业，顺利考入Caltech（加州理工学院，关于这个学校有多牛逼我相信懂得都懂）读博，数学专业，并于20岁毕业，成为当时该校有史以来最年轻的女博士（也是学数学）。
我有幸和她坐在同一间教室听课，也是因为她获得博士学位后，心血来潮回母校看看老师。当时坐在大神后面每天都很激动，我一直称她为偶像
后来我就再也没有看到她的任何信息了。刚和虎虎提到她的时候，心血来潮上网一查，发现她在获得数学博士学位后，一直在做关于核磁共振的研究，没想到她竟然在医学领域越走越远，直接考入了UCLA医学院（可以说是顶尖医学院之一）读医去了，现在已经是一名医生了！喜提MD PhD双title！
太太太太牛了！感觉我就像是读了一本爽文一样此时此刻好激动好感动，谁懂🙉！
（图3是她当时回我的email，我当时下课时问了她一个我想不明白的微积分相关的知识（现在已经完全不记得了），她想了想然后说回家email给我，我当时以为只是客套话，结果没想到两天后收到她很认真的讲解，瞬间解开了我的疑惑，我回送了颗巧克力答谢。真就是那种”another story to tell your grandchildren”） source
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