NEM reacts almost exclusively with Cys residues, and modification of 1-2 Cys per tubulin monomer
做了17页slides（非完稿版（大概一半proposal的内容（但是是简单的那一半
感觉昨天太overwhelmed了于是今天整个人都不太好一直昏昏沉沉
一点也没有尽头的准备quals的日子QAQ
倒计时10-7
感觉昨天太overwhelmed了于是今天整个人都不太好一直昏昏沉沉
一点也没有尽头的准备quals的日子QAQ
倒计时10-7
The chromosomal passenger complex (CPC) is a 'master controller' of cell division that is formed by a kinase module (Aurora B kinase) and a localization module (the scaffolding protein inner centromere protein (INCENP), survivin and borealin).
https://www.nature.com/articles/nrm3474
https://www.nature.com/articles/nrm3474
Nature
The chromosomal passenger complex (CPC): from easy rider to the godfather of mitosis
Nature Reviews Molecular Cell Biology - The chromosomal passenger complex (CPC), which is formed by inner centromere protein (INCENP), borealin, survivin and Aurora B kinase, targets to different...
We propose that MT plus-end-associated centralspindlin recruits a cortical pool of Dm ECT2 upon physical contact to activate RhoA
https://elifesciences.org/articles/38968
https://elifesciences.org/articles/38968
eLife
Microtubule plus-ends act as physical signaling hubs to activate RhoA during cytokinesis
MT plus-end-mediated recruitment of a cortical pool of ECT2 trigger RhoA activation upon contact, which results in localized contractility during cytokinesis.
elective disruption of microtubule organization revealed that both astral and central spindle microtubules can recruit RhoA at the equatorial cortex.
https://pubmed.ncbi.nlm.nih.gov/16352658/
https://pubmed.ncbi.nlm.nih.gov/16352658/
PubMed
Centralspindlin regulates ECT2 and RhoA accumulation at the equatorial cortex during cytokinesis - PubMed
During determination of the cell division plane, an actomyosin contractile ring is induced at the equatorial cell cortex by signals from the mitotic apparatus and contracts to cause cleavage furrow progression. Although the small GTPase RhoA is known to regulate…
It can be used to simulate forces that can be exerted at a cellular or a subcellular level
what we can have is filments and associated molecules, like molecular motors or cross linkers that interact with these filaments and exert forces even at a nanometer scale and we can see how these forces propagate over tens of micrometers
it simulates filaments based on connected segments, and you have some associated molecules which can interact with these cytoskeletal elements.
https://www.embl.org/training/self-paced-learning/simulating-cytoskeleton-cytosim/#:~:text=Cytosim%20is%20an%20accessible%20simulation,up%20to%20a%20few%20cells.
what we can have is filments and associated molecules, like molecular motors or cross linkers that interact with these filaments and exert forces even at a nanometer scale and we can see how these forces propagate over tens of micrometers
it simulates filaments based on connected segments, and you have some associated molecules which can interact with these cytoskeletal elements.
https://www.embl.org/training/self-paced-learning/simulating-cytoskeleton-cytosim/#:~:text=Cytosim%20is%20an%20accessible%20simulation,up%20to%20a%20few%20cells.
www.embl.org
Simulating the Cytoskeleton Using Cytosim
Cytosim is an accessible simulation software – developed by EMBL scientists – to simulate realistic individual elements of the cytoskeletal network.
To position the division plane, the microtubule-rich mitotic spindle communicates with the cell cortex via Rho GTPase-mediated signal transduction pathways to locally activate the assembly and constriction of the actomyosin contractile ring at the cell equator
水水的摸鱼日常
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4245201/#R19
Active myosin-II binds to diaphanous-related formin-generated actin filaments and drives constriction of these filaments, much like the sliding filament model defining the contraction of muscle cells.