水水的摸鱼日常
@changeless
2.3K subscribers
3.33K photos
20 videos
49 files
2.08K links
自用mark
频道所有转发完全不代表本人观点

菜得一逼的鶸鸡生物搬砖狗的搬砖日常
博士四年级在读 quit倒计时ing
除了生物什么都做

做过Archaea，UAA，CRISPR-Cas，evolution，microbiome，barcode，RNA regulation，imaging，dev bio

Paper/Seminar Record: https://t.me/PaperReading
My daily crap: https://t.me/loveuchangeless
Download Telegram
About
Blog
Apps
Platform
Join
水水的摸鱼日常
2.3K subscribers
水水的摸鱼日常
115 views
水水的摸鱼日常
108 views
水水的摸鱼日常
107 views
水水的摸鱼日常
109 views
水水的摸鱼日常
117 views
水水的摸鱼日常
122 views
水水的摸鱼日常
https://www.plasmidsaurus.com/faq/
122 views
水水的摸鱼日常
干了一早上
结果新microscope miss a part bc they forgot to pack it
……
…………
………………
123 views
水水的摸鱼日常
一天生产10G+的垃圾
140 views
水水的摸鱼日常
👍2
167 views
水水的摸鱼日常
https://mp.weixin.qq.com/s/tLp-kOAERzOW82hRR2yyaA
345 views
水水的摸鱼日常
Forwarded from 每个女孩都是最美的女孩
“紫色的大丽菊”
👍2
112 views
水水的摸鱼日常
https://mp.weixin.qq.com/s/zNXdgn-VqGN1N1R9j-6m2A?v_p=90&WBAPIAnalysisOriUICodes=10000001_10000002&launchid=default&wm=3333_2001&aid=01A1R2NZRukHqPTs_yI47Ry3ZMMdZn-y2tQqokUctvK6uGwMc.&from=10C8093010
130 views
水水的摸鱼日常
Forwarded from Web to Album
#bioart分享# ——【Cell评述 | 组织#生物打印#新进展】

2022年7月21日，加拿大维多利亚大学的Mohsen Akbari和美国寺崎生物医学创新中心的Ali Khademhosseini合作在#Cell#杂志发表评述文章#Tissue bioprinting for biology and medicine#，讨论该技术未来的挑战，以便更广泛地应用于生物学和医学领域。#科普# Cell评述 | 组织生物打印新进展 source
122 views
水水的摸鱼日常
https://blog.addgene.org/plasmids-101-the-promoter-region#:~:text=The%20sequence%20of%20the%20promoter,of%20interest%20will%20be%20expressed.
blog.addgene.org
Plasmids 101: The Promoter Region – Let's Go!
The next post in the Plasmids 101 series focuses on the promoter region, the element responsible for initiating the transcription of your insert into RNA.
163 views
水水的摸鱼日常
水水的摸鱼日常
https://blog.addgene.org/plasmids-101-the-promoter-region#:~:text=The%20sequence%20of%20the%20promoter,of%20interest%20will%20be%20expressed.
113 views
水水的摸鱼日常
https://en.wikipedia.org/wiki/Tac-Promoter#:~:text=The%20Tac%2DPromoter%20(abbreviated%20as,protein%20production%20in%20Escherichia%20coli.
Wikipedia
Tac-Promoter
The Tac-Promoter (abbreviated as Ptac), or tac vector is a synthetically produced DNA promoter, produced from the combination of promoters from the trp and lac operons. It is commonly used for protein production in Escherichia coli.
131 views
水水的摸鱼日常
https://www.researchgate.net/post/Protein-expression-differences-T7-vs-tac-promoter
ResearchGate
Protein expression differences T7 vs tac promoter
I've been testing the expression of certain protein N-term tagged GST/Ct-term Histag in E.coli BL21 DE3 pLysS that is quite susceptible to proteolytic cleavage so has to be extracted after 3 hours...
114 views
水水的摸鱼日常
fmicb-05-00172.pdf
764.6 KB
118 views
水水的摸鱼日常
https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/cloning-and-expression/tac-promoter-system
126 views
水水的摸鱼日常
https://www.researchgate.net/post/Can-T7-RNA-polymerase-bind-to-trc-promoter-in-a-vector-in-Ecoli
ResearchGate
Can T7 RNA polymerase bind to trc promoter in a vector in E.coli ?
I was reading a paper that used escherichia coli origami DE3 strains to express a certain protein and the vector used was a pTrcHis-Topo with a trc promoter. I am wondering if they made use of the...
123 views