水水的摸鱼日常

141 views15:39

水水的摸鱼日常

https://www.cytoskeleton.com/tl590m

141 views15:59

草原来是老板偷了我从隔壁lab搞来的小号ice bucket
我找了好久了！！我好气啊.jpg

141 views16:08

水水的摸鱼日常

老板：为什么不work啊？？？？
我：老板啊我真的觉得是你的那管试剂不functional了
我OS：特么你都走了两个多小时了试剂就这么随便放冰上不管也不知道什么时候会回来管你问我为什么不work个锤子啊？能work才奇怪了好吗我辛辛苦苦纯化的蛋白就这么被你糟蹋啊喂

145 views18:31

水水的摸鱼日常

美

148 views18:31

水水的摸鱼日常

1.avi

15.5 MB

生物好美哦

149 views18:43

水水的摸鱼日常

哭了
上周这周下周都有校内的同届小伙伴去conference
我也想去conference啊大哭（主要想去玩

188 views20:10

水水的摸鱼日常

Forwarded from Web to Album

【饶毅说过他当时（申请学校时）有三封美国科学院院士推荐信，都是来给讲座的时候认识的。】——整个人精神都不好了，只能羡慕嫉妒恨地脑补饶毅有洗脑术了，瞅一瞅对方就迷倒了。。。。 source

162 views22:20

水水的摸鱼日常

这是什么training?

139 views17:35

水水的摸鱼日常

https://www.neb.com/tools-and-resources/video-library/do-i-need-a-5-phosphate-for-ligation

Neb

Quick Tips - Do I need a 5' phosphate for ligation? | NEB

This video provides tips for phosphorylating fragments prior to performing a ligation reaction.

139 views19:48

水水的摸鱼日常

131 views23:36

水水的摸鱼日常

https://www.researchgate.net/post/Composition_of_sonication_buffer_for_bacterial_cell_lysis

ResearchGate

Composition of sonication buffer for bacterial cell lysis ?

What are the composition of sonication buffer for bl21 de3 containing his tag proteins?

127 views00:10

水水的摸鱼日常

https://www.aatbio.com/resources/faq-frequently-asked-questions/Why-is-EDTA-used-in-lysis-buffer

130 views00:17

水水的摸鱼日常

https://www.sheffield.ac.uk/polopoly_fs/1.779623!/file/PurificationGuide.pdf

129 views00:26

水水的摸鱼日常

请问：
用E.coli提纯蛋白，在harvest了e.coli后立刻提纯或者-80冻几天再提纯的区别很大吗？
为什么我每次冻后感觉可以帮助cell lysis啊

120 viewsedited 00:34

水水的摸鱼日常

https://www.researchgate.net/post/Do-you-snap-freeze-your-cells-and-make-cell-lysates-later-to-check-phospho-proteins-by-western-blot

ResearchGate

Do you snap freeze your cells and make cell lysates later to check...

I am working with leukemia/floating cells. Typically, I spin down the cells, wash them with ice-cold PBS, spin again and then lyse the cells immediately.
I am thinking If you could snap freeze the...

122 views01:28

水水的摸鱼日常

https://www.neb.com/faqs/0001/01/01/how-much-dna-should-be-used-in-a-ligation-using-t4-dna-ligase

Neb

How much DNA should be used in a ligation using T4 DNA Ligase? | NEB

The unit definition uses 0.12 μM (300 μg/ml) lambda HindIII fragments. The high DNA concentration can be used for linker ligation. To promote circle formation, useful in transformation, a lower total DNA concentration should be used.

125 views02:56

水水的摸鱼日常

Forwarded from 糟糕！要失学了！

阔佬朋友带带咱

https://mp.weixin.qq.com/s/odvykDEoqxMsJVpBOW_sZg

104 views03:53

水水的摸鱼日常

Forwarded from Web to Album

2020年持非移民签证在美国获得博士学位的有一万八千多人，其中中国国籍的有六千三百多人。这两个数字我乍一看都觉得有点少？不过想想美国提供phd的学校就那么多所，算算好像也正常 source

127 views05:01

水水的摸鱼日常

116 views21:58

水水的摸鱼日常

115 views00:00

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