Base editing vs. prime editing (David Liu)
https://www.liugroup.us/research-overview/genome-editing/
"Base editing: In 2016 the Liu group developed base editing, a genome editing method that efficiently converts one base pair to a different base pair without inducing double-stranded DNA breaks or extensive insertions and deletions (indels). The two classes of base editors (adenine base editors or ABEs and cytosine base editors or CBEs) developed by the Liu group can correct all four transition mutations, which collectively account for more than 60% of human pathogenic point mutations.
Prime editing: In 2019 the Liu group developed prime editing, a new approach to genome editing in which a reverse transcriptase directly copies edited DNA sequences into a specified target site from an extended guide RNA without requiring double-stranded DNA breaks or donor DNA templates. Prime editing is highly versatile, and can mediate all possible base-to-base conversions, insertions, deletions, and combinations thereof, at target locations near or far from PAM sequences"
https://www.liugroup.us/research-overview/genome-editing/
"Base editing: In 2016 the Liu group developed base editing, a genome editing method that efficiently converts one base pair to a different base pair without inducing double-stranded DNA breaks or extensive insertions and deletions (indels). The two classes of base editors (adenine base editors or ABEs and cytosine base editors or CBEs) developed by the Liu group can correct all four transition mutations, which collectively account for more than 60% of human pathogenic point mutations.
Prime editing: In 2019 the Liu group developed prime editing, a new approach to genome editing in which a reverse transcriptase directly copies edited DNA sequences into a specified target site from an extended guide RNA without requiring double-stranded DNA breaks or donor DNA templates. Prime editing is highly versatile, and can mediate all possible base-to-base conversions, insertions, deletions, and combinations thereof, at target locations near or far from PAM sequences"
水水的摸鱼日常
日了狗了 今天又跑了一只 我觉得我和青蛙大概有仇 我不想再做青蛙实验了
找回来了一只！（另外一只大概永远的消失了
可惜单独养了一周末，看起来还是没有fully recover。。。喂了点吃的也不是很感兴趣的亚子
可惜单独养了一周末，看起来还是没有fully recover。。。喂了点吃的也不是很感兴趣的亚子
The mechanism of pH meter/electrode
https://www.ysi.com/ysi-blog/water-blogged-blog/2pH19/02/anatomy-of-ph-electrodes
https://www.ysi.com/ysi-blog/water-blogged-blog/2pH19/02/anatomy-of-ph-electrodes