why HEPES? In general, the optimal culture environment for most cells is pH 7.2-pH 7.4 [1][2], and the buffering range of HEPES falls exactly in pH 6.8-pH 8.2, which is favorable for cell culture. Compared with other buffers such as PBS (phosphate buffered saline) and TRIS, HEPES has higher stability in maintaining the pH values of the cell culture media[3][4][5], that’s also the reason why HEPES is widely used in cell culture , tissue culture, protein purification and extraction, immunoprecipitation, cell lysis, live cell imaging and other biological/biochemical researches.
https://www.hopaxfc.com/en/blog/why-use-hepes
https://www.hopaxfc.com/en/blog/why-use-hepes
Hopax Fine Chemicals
Why use HEPES? - Hopax Fine Chemicals
Compared with other buffers such as PBS (phosphate buffered saline) and TRIS, HEPES has higher stability in maintaining the pH values of the cell culture media, that’s also the reason why HEPES is widely used in cell culture
https://mp.weixin.qq.com/s/_eG8urgRV_9yP33J0x7Nlw
发现肥胖导致的代谢紊乱能诱导小鼠毛囊干细胞内炎症信号的激活以及ROS与脂类的异常积累，进而通过抑制Sonic Hedgehog（SHH）通路诱发毛囊干细胞耗竭，最终导致毛发减少。
“肥胖让人变秃”
发现肥胖导致的代谢紊乱能诱导小鼠毛囊干细胞内炎症信号的激活以及ROS与脂类的异常积累，进而通过抑制Sonic Hedgehog（SHH）通路诱发毛囊干细胞耗竭，最终导致毛发减少。
“肥胖让人变秃”
#佛山市仙湖实验室#面向全球诚邀海外青年学者专家加入！
广东省省级重点实验室科研平台，致力于氢能、燃料电池等新能源、新材料研究，现因研究需要，招聘海外专家、教授、博士人才；研究方向如下（包含但不限于）：
1️⃣电子皮肤、柔性传感器、柔性机器人等；
2️⃣物理或光学，超快泵浦探测或激光微纳加工等；
3️⃣材料类或者化学、化工专业；研究方向为电池类、高分子类、纳米材料类、计算类；工程技术人员（硕士）：电池类或薄膜或涂布类；
4️⃣控制科学与工程专业；
5️⃣流体和力学模拟仿真，能源与动力学院相关专业；
仙湖实验室将协助申报国家优青、青年人才等项目，国家与佛山市政府双重人才引进补贴，绝佳科研环境，薪资待遇丰厚，青年人才90万/年，研究员60-100万/年，领军人才110万/年，另额外享有政府补贴，福利优渥；欢迎您的加入！
📮应聘方式：
个人简历发送至邮箱：xhlab@group.zhaopin.com.cn
如有疑问，可点击链接，填写登记表：http://sqmdbenzpdmpkpww.mikecrm.com/58bl9eb
实验室官网：http://www.xianhulab.com
广东省省级重点实验室科研平台，致力于氢能、燃料电池等新能源、新材料研究，现因研究需要，招聘海外专家、教授、博士人才；研究方向如下（包含但不限于）：
1️⃣电子皮肤、柔性传感器、柔性机器人等；
2️⃣物理或光学，超快泵浦探测或激光微纳加工等；
3️⃣材料类或者化学、化工专业；研究方向为电池类、高分子类、纳米材料类、计算类；工程技术人员（硕士）：电池类或薄膜或涂布类；
4️⃣控制科学与工程专业；
5️⃣流体和力学模拟仿真，能源与动力学院相关专业；
仙湖实验室将协助申报国家优青、青年人才等项目，国家与佛山市政府双重人才引进补贴，绝佳科研环境，薪资待遇丰厚，青年人才90万/年，研究员60-100万/年，领军人才110万/年，另额外享有政府补贴，福利优渥；欢迎您的加入！
📮应聘方式：
个人简历发送至邮箱：xhlab@group.zhaopin.com.cn
如有疑问，可点击链接，填写登记表：http://sqmdbenzpdmpkpww.mikecrm.com/58bl9eb
实验室官网：http://www.xianhulab.com
Mikecrm
意向登记表
佛山仙湖实验室2021海外人才引进项目
https://mp.weixin.qq.com/s/U3UVWngh1zO2VIciMJKwuA
https://mp.weixin.qq.com/s/_gkiNu9dEztJSINDRUuStw
https://www.eurekalert.org/pub_releases/2021-06/caos-nfu062421.php
唉 我愿意把付巧妹的成功 称为天时地利人和俱全
太厉害了
首先博士期间刚好和组里一起发了一篇关于如何用现代人基因作为磁铁吸附&富集土壤里即使只有0.03%的古代人基因的方法
然后从德国回到中国，作为第一人，用这个方法把中国各个地方的古代人基因（包括mtDNA）都研究了一遍，发了好多的CNS
真的是天时地利人和
刚好就是她了.jpg
https://mp.weixin.qq.com/s/_gkiNu9dEztJSINDRUuStw
https://www.eurekalert.org/pub_releases/2021-06/caos-nfu062421.php
唉 我愿意把付巧妹的成功 称为天时地利人和俱全
太厉害了
首先博士期间刚好和组里一起发了一篇关于如何用现代人基因作为磁铁吸附&富集土壤里即使只有0.03%的古代人基因的方法
然后从德国回到中国，作为第一人，用这个方法把中国各个地方的古代人基因（包括mtDNA）都研究了一遍，发了好多的CNS
真的是天时地利人和
刚好就是她了.jpg
Forwarded from Paper/Seminar Record (百合子)
6/25
Human population history at the crossroads of East and Southeast Asia since 11,000 years ago (cell 2021)
They used ancient DNA (aDNA) to reveal the dynamic changes of different groups of ancestors.
The aDNA extraction technique is developed also by Qiaomei Fu and her team when she was a PhD student in 2013 which can make modern people’s DNA into a magnet-like primer to absorb and enrich ancient people’s genes in soil even the ancient genes in the soil is only 0.03%.
They suggested Guangxin province served as an important genetic exchange junction point for both North East Asian and Southeast Asian groups.
Her group also published another paper using the same dataset but instead of using nuclear gene for analysis, the other is using mtDNA information. (https://www.sciencedirect.com/science/article/abs/pii/S1673852721001703?via%3Dihub)
Human population history at the crossroads of East and Southeast Asia since 11,000 years ago (cell 2021)
They used ancient DNA (aDNA) to reveal the dynamic changes of different groups of ancestors.
The aDNA extraction technique is developed also by Qiaomei Fu and her team when she was a PhD student in 2013 which can make modern people’s DNA into a magnet-like primer to absorb and enrich ancient people’s genes in soil even the ancient genes in the soil is only 0.03%.
They suggested Guangxin province served as an important genetic exchange junction point for both North East Asian and Southeast Asian groups.
Her group also published another paper using the same dataset but instead of using nuclear gene for analysis, the other is using mtDNA information. (https://www.sciencedirect.com/science/article/abs/pii/S1673852721001703?via%3Dihub)
Pyroptosis 焦亡
highly inflammatory (different from Apoptosis)
Similarity with apoptosis: DNA片段化、核凝聚和caspase依赖性
The process is initiated by formation of a large supramolecular complex termed the inflammasome (also known as a pyroptosome) upon intracellular danger signals.
Finally, the cells burst and dissolve, and the cytoplasmic contents are released to the outside world
highly inflammatory (different from Apoptosis)
Similarity with apoptosis: DNA片段化、核凝聚和caspase依赖性
The process is initiated by formation of a large supramolecular complex termed the inflammasome (also known as a pyroptosome) upon intracellular danger signals.
Finally, the cells burst and dissolve, and the cytoplasmic contents are released to the outside world