水水的摸鱼日常
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频道所有转发完全不代表本人观点

菜得一逼的鶸鸡生物搬砖狗的搬砖日常
博士四年级在读 quit倒计时ing
除了生物什么都做

做过Archaea,UAA,CRISPR-Cas,evolution,microbiome,barcode,RNA regulation,imaging,dev bio

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Cycloheximide is a naturally occurring fungicide produced by the bacterium Streptomyces griseus. Cycloheximide exerts its effects by interfering with the translocation step in protein synthesis (movement of two tRNA molecules and mRNA in relation to the ribosome), thus blocking eukaryotic translational elongation. Cycloheximide is widely used in biomedical research to inhibit protein synthesis in eukaryotic cells studied in vitro (i.e. outside of organisms). - prevent the interphase extract from entering the next mitosis
Ringer's solution is a solution of several salts dissolved in water for the purpose of creating an isotonic solution relative to the body fluids of an animal. Ringer's solution typically contains sodium chloride, potassium chloride, calcium chloride and sodium bicarbonate, with the last used to balance the pH. Other additions can include chemical fuel sources for cells, including ATP and dextrose, as well as antibiotics and antifungals.
EDTA: 金属螯合剂,降低酶活性,把酶里面的金属离子螯合出来。
破坏溶液中的DNase, RNase
破坏细胞的桥粒结构,使细胞能够分散成单个细胞
HEPES/ PDS/ Tris: buffer system
Ringer's solution is a solution of several salts dissolved in water for the purpose of creating an isotonic solution relative to the body fluids of an animal. Ringer's solution typically contains sodium chloride, potassium chloride, calcium chloride and sodium bicarbonate, with the last used to balance the pH. Other additions can include chemical fuel sources for cells, including ATP and dextrose, as well as antibiotics and antifungals.
MMR buffer: Marc’s modified ringer buffer
Forwarded from Web to Album
高瓴想招一个生物PhD做投资,各种金融方面的条件都设成非必须了,还是几个月都招不到人……大家都会有光明的未来的 source
Pigment granules in Stentor coeruleus are extrusive organelles containing the pigment, stentorin, which provides the blue-green colouration to this ciliate. A small, subcellular membrane-bounded vesicle containing pigment and/or pigment precursor molecules
Forwarded from 白川 カップ Official | 靓靓杯
Cortical granules are regulatory secretory organelles (ranging from 0.2 um to 0.6 um in diameter) found within oocytes and are most associated with polyspermy prevention after the event of fertilization. Cortical granules are found among all mammals, many vertebrates, and some invertebrates. Within the oocyte, cortic
Aneuploidy is the presence of an abnormal number of chromosomes in a cell, for example a human cell having 45 or 47 chromosomes instead of the usual 46. ... An extra or missing chromosome is a common cause of some genetic disorders. Some cancer cells also have abnormal numbers of chromosomes.
https://www.zhihu.com/question/306461432
(前两个回答都写得很好)