Organic fluorophores have superior properties over fluorescent proteins such as a wider spectral range, smaller size, greater photostability, and, in many cases, higher brightness. Coupling the label to the target protein can be achieved by direct and indirect labeling methods
Organic fluorophores are not proteins - smaller than protein\
Organic fluorophores are not proteins - smaller than protein\
A region of interest (ROI) is a portion of an image that you want to filter or operate on in some way.