lower temperature & dietary restriction 是两个减慢衰老的主要方式
神奇的是：相对于lower temperature的有memory，dietary restriction是没有memory的 - 一旦你不再维持dietary restriction，你将立刻恢复正常的衰老模式（之前的努力都作废）
当然 这也意味着 什么时候开始dietary restriction都不算晚

给组里的学生一个老欧改毕业论文，然后看到这个Acknowledgements 里面这段话

你说我改不改吧。

改吧，感觉他的情绪要抒发不出来；

不改吧，到时候答辩委员会和大boss看了不得抽死我 source

HRM分析
https://www.plob.org/article/3359.html

https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/real-time-pcr-applications/genetic-variation-analysis-using-real-time/high-resolution-melting-hrm.html

Detect SNP with qPCR instrument and saturated dyes

The difference between probe and primer:
1. Probe is hybridized with ssDNA
2. Promer is hybridized with dsDNA

师姐说，早上六点半看到老板发来一封邮件，让她有空打个电话回去有事找
当时师姐就在心里冷笑一声，心想：比早？
然后反手就把电话打了回去
笑死我了哈哈哈哈

An interesting proposal:
Find a graveyard - isolate the bacteria feed off of the dead bodies - propose these bacteria have evolved enzymes - introduce them into our bodies to get rid of molecule that are accumulated with aging

senescent cells (cells that have been divided for several times) will accumulate in human body and they will contribute to the aging phenotype.

有点丑

https://www.sigmaaldrich.com/technical-documents/protocols/biology/annealing-oligos.html

Protocol for Annealing Oligonucleotides

SDS-PAGE is an electrophoresis method that allows protein separation by mass. The medium (also referred to as ′matrix′) is a polyacrylamide-based discontinuous gel. In addition, SDS (sodium dodecyl sulfate) is used. About 1.4 grams of SDS bind to a gram of protein, corresponding to one SDS molecule per two amino acids.

【在培养皿中生成人囊胚样结构】《自然》发表的两篇论文描述了两种在实验室生成的人囊胚样结构。这些结果为研究人体早期发育提供了一个模型，或能增进我们对早期发育缺陷的认识，帮助开发新的体外受精（IVF）相关疗法。

卵子在受精后的几天内会发育成囊胚。囊胚是一种球形结构，外层的细胞包裹着充满液体的内腔，腔内含有大量胚胎细胞。不过，由于缺少合适的模型，我们对人体早期胚胎发育的理解一直很有限。对IVF治疗后捐献的人囊胚的研究提供了一些见解，但这些囊胚的可及性和使用都受到了限制。近来，实验室已经生成了名为胚状体的小鼠囊胚样结构，这种胚状体可以模拟小鼠早期发育的多个方面，但之前没有研究报道过使用人体细胞生成的类似胚状体。

澳大利亚莫纳什大学克雷顿校区的Jose Polo和同事重编程了人成纤维细胞――结缔组织的主要细胞类型――从而在实验室构建了人囊胚的三维模型，并称其为“iBlastoids”（诱导胚状体）。作者发现，iBlastoids不仅能模拟囊胚的整体结构，还能支持多能干细胞和滋养层干细胞的生长。iBlastoids也可以模拟早期着床的多个方面。不过，他们指出iBlastoids不应被看作与人囊胚是等价的。网页链接

在另一项研究中，美国达拉斯得克萨斯大学西南医学中心的吴军和同事开发了一种能从人多能干细胞得到囊胚样结构的三维培养方法，他们将该结构称为“人胚状体”。这种人胚状体在形态、大小、细胞数量、不同细胞系的构成方面都类似于人囊胚。人胚状体能产生胚胎干细胞和胚外干细胞，并能自组织成具有围着床期人胚胎特征的结构。此外，作者还发现了蛋白激酶C在胚状体腔形成中的信号转导作用。作者强调人胚状体与人囊胚并不等价，也无法发育成可存活的胚胎。网页链接 source

最近好拖延
有个事情拖到现在才做
（其实也不难做，就是感觉很反感去做，宁愿去做别的很复杂的事情也不想碰不想做的事情
科研好难啊.jpg

Antagonistic Pleiotropy (AP) is today the best-accepted theory for the evolutionary origin of aging. According to AP theory, aging is a side effect of genes that are selected for their contribution to fertility and other essential components of individual fitness. In this standard view, aging exists because the benefits of enhanced fertility early in life are linked logically or physically to the long-term deterioration of the body, and evolution has been compelled to accept the latter as a cost of the former.

DOI: 10.1134/S0006297919120058
What Is Antagonistic Pleiotropy?

How to do deep-learn the FPLC (superdes)
1. Filter – change to a new filter
2. Beads
a. Organic solvents – wash off hydrophobic molecule
b. H2O
c. NaOH 0.5M
d. H2O
e. Acid - acetic acid
f. H2O
2. Exceed pressure limit – the resin will be compressed
3. Repack column – need two columns

Difference between FPLC and HPLC

HPLC: purify protein of small volume with a higher precision

FPLC: Fast protein liquid chromatography – used for purification for protein of large volume

我的PS也太学术了一点（