Acetone is one type of Cleaning and is Fast but a fire hazard

In impregnation the tissues are put from 2 - 4 hours in hot soft paraffin at 57 °C

Trimming is should be immersed in cold water to cool it rapidly

Paraffin blocks are cut by microtome using metal knife
into thin sections

When using Microtome must to Put the scale at 20 microns which indicate the thickness of the sections.

The knife is sharp and tilted to a suitable angle.

The ribbon is floated on the water surface heated at

Microtome is a technique used to enhance contrast
in samples.

Histological studies are used in

Staining is the most common staining technique in histopathology.

Eosin stains the parts of the cell which are basophilic

Hematoxylin stains the parts of the cell which are