Southern blot steps:
1- extraction of DNA
2- DNA cleaved into many fragments
3- Separation of fragments based on size by electrophoresis
4- DNA fragments in gel are denatured into ssDNA & transferred (blotted) to a nitrocellular membrane.
5- Gene of interest is only 1 or a few fragments.
6- Usage of a probe to identify the fragments of interest.
هذه الخطوات من ليبينكوت لعلّها تفيدكم.
1- extraction of DNA
2- DNA cleaved into many fragments
3- Separation of fragments based on size by electrophoresis
4- DNA fragments in gel are denatured into ssDNA & transferred (blotted) to a nitrocellular membrane.
5- Gene of interest is only 1 or a few fragments.
6- Usage of a probe to identify the fragments of interest.
هذه الخطوات من ليبينكوت لعلّها تفيدكم.
SoMA
BC243 Regulation of G.E in eukaryotes_20190127212403.pdf
تفريغ محاضرات Regulation of gene expression in eukaryotes.
SoMA
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