With the increased focus on reproducibility of scientific data, it is important to look at how data is interpreted. To assist in data interpretation, the scientific method requires that controls are built into the experimental workflow. These controls are…

3 different theories on compensation are discussed. The first, non-pensaton, is not recommended, and only possible under a narrowly defined instrument. The second, manual compensation, is also not recommended for anything more than 2 fluorochromes. It is…

There is a lot of preparatory work that must be done before the first flow cytometry experiment can be attempted. Each step builds upon the previous one and extends where the assay is going. Be prepared for some trial and error in this process, and don’t…

After completing the perfect staining and cytometry run, the hard work begins – data analysis.  To properly identify the cells of interest, it is critical to pull together knowledge of the biology with the controls run in the experiment to properly place…