🕳سوال (يوم الاحد)🕳
السوال الثاني

معانا طبقين B.A and Macc.A مزرع عليه عينه urine
🕳B.A →Non growth
🕳Macc.Agar→ appear growth colony gram negative bacteria
large coloy
raised colony
gray-white colony

ماذا يدل هذا النمو بالطبقين ؟
🔝عشاق الميكرو😉

للاجابه رسلها هنا
@AANMLab7bot
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🔝اجابة السوال 🔝
💢 لماذا يفضل استخدام مزرعة CLED في عزل عينة urine؟؟

لان هذه المزرعة CLED يعطي نتائج دقيقة للاسباب عديدة منها:-
الاول:-

❇️allow growth Gram positive Gram Negative pathogenic bacteria
يسمح بنمو البكتيريا الموجبه والسالبه

❇️ثانيا :- أيضا لأن الCLEDيستخدم في عزل وتفريق بكتيريا المسالك البوليه
❇️أيضا يمكنه التمييز بين البكتيريا المخمره للاكتوز وغير المخمره
Lactose use to diffrentiat Gram negative bacteria to Lactose fermention
non lactose fermention
ثالثا :- electrolyte deficient الموجود في المزرعة تعمل على منع swarming لبكتيريا البروتيس بسبب الالكتروليت الموجود في البيئه
❇️Prevent the swarming of proteus in media
🔝 وفي حاله عدم توفر CLED ممكن نستخدم ال Blood agar & Mac للتزريع مع بعض وليس شرطا وجود ال cled
وتحتوى CLED على كاشف Bromo-thymol blue
كما انها تعتبر لها نفس وظيفة الـ Agar MacConkey
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#منتدى_الطب_المخبري
د. عبدالله احمد

🔝اجابة السوال 🔝
Contamination
🧪لانه معروف ان بيئه Blood Agar تعتبر بيىة غنية بالمواد الغذائيه التي تسمح بنمو البكتيريا الموجبه والسالبه ماعدا البكتيريا المدلله التي تختاج الى عامل v, factor x factor وعدد اخر
وتعتبر اجار الدم والماكونكي هنا تكون تاكيديه

🧪اما اجار الدم لايوجد فيها نمو والماكونكي نمو معناه تلوث Contamination

ونمو البكتيريا في وسط الماكونكي مهما كان نوعها وغيابها في اجار الدم هنا تلوث اعتبرها مهما كانت نوع البكتيريا في الماكونكي لان غيابها في B. A يعتبر تلوث
د.عبدالله احمد
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Tran-isolate medium is used to inoculate and transport CSF sample, If the CSF cannot be transported to a microbiology laboratory immediately (within 1 hour from the time of collection) for culture and analysis of cerebrospinal fluids from patients with bacterial meningitis. Trans-Isolate medium is a diphasic medium, consisting: Slant: Charcoal-starch agar Broth: Soybean-casein digest-gelatin broth buffered at pH 7.2 with 0.1 M 3-(N-morpholino) propanesulfonic acid buffer. Fig. Trans Isolate (T-I) Medium T-I medium supports the growth and survival of Neisseria meningitidis, Streptococcus pneumoniae, and Haemophilus influenza. In case of stock culture T-I medium supported the growth for at least 3 months.

Tran-isolate medium is used to inoculate and transport CSF sample, If the CSF cannot be transported to a microbiology laboratory immediately (within 1 hour from the time of collection) for culture and analysis of cerebrospinal fluids from patients with bacterial meningitis. Trans-Isolate medium is a diphasic medium, consisting: Slant: Charcoal-starch agar Broth: Soybean-casein digest-gelatin broth buffered at pH 7.2 with 0.1 M 3-(N-morpholino) propanesulfonic acid buffer. Fig. Trans Isolate (T-I) Medium T-I medium supports the growth and survival of Neisseria meningitidis, Streptococcus pneumoniae, and Haemophilus influenza. In case of stock culture T-I medium supported the growth for at least 3 months.

Procedure for primary culture from Trans-Isolate (T-I) medium Remove the aluminum cap of Trans-isolate medium with forceps Wipe the rubber stopper with a 70% alcohol swab (do not use povidone-iodine as it may be carried into the medium by the passing needle, thus inhibiting the growth of bacteria.) Aseptically inoculate 0.5-1.0 ml of the CSF into the T-I medium with the use of syringe Incubate the inoculated T-I medium at 35-37°C with ~5% CO2 (or in a candle-jar) overnight or until transport is possible If the T-I medium can be transported to a microbiology laboratory the same day of inoculation, do not vent the T-I bottle until it arrives in the receiving laboratory. Upon arrival, wipe the rubber stopper with 70% alcohol, insert a venting needle into the T-I bottle, incubate at 35-37°C with ~5% CO2 (or in a candle-jar), and observe daily for turbidity in the liquid phase for up to 7 days. Prior to subculture, remove the venting needle and wipe the rubber stopper with 70% alcohol. Use a sterile needle and syringe to transfer 50-100 µl of the liquid portion of the T-I medium onto both a Blood agar Plate (BAP) and Chocolate agar (CAP) for primary culture. Approximately 50-100 µl is used to streak each plate. To streak two plates, draw approximately 100-200 µl with the syringe at one time to minimize the possibility of contaminating the T-I medium. Streak the BAP and CAP for isolation, incubate the plates at 35-37°C with ~5% CO2 (or in a candle-jar), and examine the plates daily for up to 72 hours. If no growth is observed, subculture the T-I medium again on day 4 and day 7. Isolates should always be inspected for purity of growth by looking at colony morphology before any testing is performed. If any contamination is seen, cultures should be re-streaked to ensure purity prior to testing.

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🔝EMB media for E.coli
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🔝EMB media for E.coli
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🔝EMB media for E.coli
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🔝EMB media for E.coli
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🔝EMB media for E.coli
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🔝EMB media for E.coli
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🔝EMB media for E.coli
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🔝EMB media for E.coli
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🔝EMB media for E.coli
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🔝EMB media for E.coli
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🔝Trans isolate medium
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