Medical laboratory science
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Msr x who is working at molecular laboratory was planning to observe the image of DNA bands on a gel electrophoresis from the result of PCR amplicons of patients samples with the help of UV light. He forgot to add Ethidium Bromide to a DNA solution.
Anonymous Quiz
58%
A. No bands will be seen irrespective of patients infection status
25%
B. Positive sample will show bands on the gel
13%
C. Negative samples will have no bands on the gel
4%
D. The ladder bands will be visible
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A molecular biologist who is working at a regional laboratory was running PCR for the diagnosis of infectious diseases and wanted to monitor PCR product accumulations while the PCR is still running. Which type of PCR can satisfy the need of this guy?
Anonymous Quiz
22%
A. Multiplex PCR
9%
B. Nested PCR
64%
C. Real-time PCR
5%
D. Qualitative PCR
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A molecular biologist was running a PCR that involved use of several pairs of primers to amplify more than one DNA fragment in the same reaction to diagnose for enteric pathogens in stool samples. What type of PCR was most likely used?
Anonymous Quiz
13%
A. Reverse transcriptase-PCR
8%
B. Nested PCR
69%
C. Multiplex PCR
10%
D. Real time PCR
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A medical laboratory scientist working at regional molecular biology laboratory forgot adding a primer to a master mix while running PCR amplification of a known segment of DNA. What would happen to PCR result?
Anonymous Quiz
11%
A. No problem with PCR amplification
64%
B. No amplification reaction
19%
C. Few amplification of segment of interest
6%
D. Few non-specific amplifications
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A medical laboratory scientist is preparing a PCR master mix to amplify DNA from a patient sample. Which enzyme is responsible for synthesizing new DNA strands during the extension step?
Anonymous Quiz
21%
Options: A. DNA ligase
48%
B. Taq polymerase
22%
C. Reverse transcriptase
9%
D. Helicase
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Forwarded from Medical Lab Academy
ADDIS_ABABA_CITY_Health_Bureau_MEDICAL_LABORATORY_EXAM_MEDIUM_WATERMARK1.pdf
738.5 KB
Addis Ababa City Administration Health Bureau Medical Lab Exam.
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