Medical laboratory science
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Semen analysis is one of the most important laboratory investigations used to evaluate male fertility and reproductive health. It involves the examination of semen to assess sperm count, motility, morphology, and other physical and microscopic characteristics.


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πŸ“Œ Definition

Semen analysis is a laboratory test performed to examine the quality and quantity of semen and sperm cells in order to evaluate male reproductive function.


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🎯 Objectives of Semen Analysis

βœ… To evaluate male infertility
βœ… To determine sperm count and motility
βœ… To assess sperm morphology
βœ… To detect infections in the reproductive tract
βœ… To confirm success of vasectomy
βœ… To evaluate reproductive gland function


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🧬 Composition of Semen

Semen is composed of:

Spermatozoa from testes

Seminal vesicle secretions

Prostatic fluid

Bulbourethral gland secretions



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πŸ“‹ Patient Preparation

Proper patient preparation is essential for accurate results.

Instructions to the Patient.

Maintain sexual abstinence for 2–7 days.

Avoid alcohol and smoking before the test.

Collect the sample in a sterile container.

Do not use lubricants or condoms.

Deliver the sample to the laboratory within 30–60 minutes.

Entire semen sample should be collected.



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πŸ§ͺ Collection of Semen Sample

Method of Collection

The semen sample is usually collected by:

βœ… Masturbation into a sterile wide-mouthed container

Precautions

Container must be clean and sterile.

Sample should be kept at body temperature.

Label the container properly.

Avoid contamination.



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πŸ” Examination of Semen

Semen examination includes:

1. Physical Examination


2. Microscopic Examination


3. Chemical Examination




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1️⃣ Physical Examination

A. Volume

The total amount of semen ejaculated is measured.

Normal Value

βœ… 1.5 – 6 mL

Clinical Significance

Low volume β†’ Obstruction or hormonal deficiency

High volume β†’ Infection or inflammation



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B. Color

Normal Color

βœ… Grayish white or opalescent

Abnormal Findings

Yellow β†’ Infection

Red/Brown β†’ Blood in semen

Clear β†’ Low sperm count



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C. Odor

Normal

Semen has a characteristic chlorine-like odor.

Abnormal odor may indicate infection.


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D. Viscosity

Fresh semen is thick and coagulated initially.

Normal

Liquefies within 15–30 minutes

Delayed liquefaction may indicate prostate gland disorder.


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E. pH

Measured using pH paper.

Normal Range

βœ… 7.2 – 8.0

Significance

Acidic pH β†’ Seminal vesicle obstruction

Alkaline pH β†’ Infection



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2️⃣ Microscopic Examination

A. Sperm Count

Determined using a hemocytometer or automated analyzer.

Normal Value

βœ… 15 million sperm/mL or more

Abnormal Conditions

Oligospermia β†’ Low sperm count

Azoospermia β†’ Absence of sperm

Polyzoospermia β†’ Excessive sperm count



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B. Sperm Motility

Motility indicates the movement ability of sperm.

Types of Motility

1. Progressive motility


2. Non-progressive motility


3. Immotile sperm



Normal Value

βœ… At least 40% motile sperm

Clinical Importance

Low motility is called:

➑️ Asthenozoospermia


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C. Sperm Morphology

Shape and structure of sperm are examined microscopically.

Normal Sperm Structure

Oval head

Intact midpiece

Long tail


Abnormal Forms

Double head

Bent tail

Large or small head


Normal Value

βœ… 4% or more normal forms

Abnormal morphology is called:

➑️ Teratozoospermia


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D. Viability Test

Determines live and dead sperm percentage.

Normal Value

βœ… 58% or more live sperm

Dead sperm condition is called:

➑️ Necrozoospermia


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E. Other Cells

Presence of:

Pus cells β†’ Infection

RBCs β†’ Bleeding

Epithelial cells β†’ Contamination

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3️⃣ Chemical Examination

Chemical tests may include:

Fructose test

Zinc estimation

Acid phosphatase


These help evaluate accessory gland function.


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πŸ“„ Example of Normal Semen Report

Parameter Normal Value

Volume 1.5 – 6 mL
Color Grayish white
pH 7.2 – 8.0
Liquefaction Time 15 – 30 min
Sperm Count β‰₯15 million/mL
Motility β‰₯40%
Morphology β‰₯4% normal forms
Viability β‰₯58% live sperm



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⚠️ Important Terminologies

Term Meaning

Oligospermia Low sperm count
Azoospermia No sperm present
Asthenozoospermia Poor sperm motility
Teratozoospermia Abnormal sperm morphology
Necrozoospermia Dead sperm cells present



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🩺 Clinical Significance of Semen Analysis

Semen analysis helps in:

βœ… Diagnosis of male infertility
βœ… Evaluation of reproductive gland function
βœ… Detection of genital tract infection
βœ… Monitoring fertility treatment
βœ… Post-vasectomy evaluation


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❌ Causes of Abnormal Semen Analysis

Smoking and alcohol

Hormonal imbalance

Varicocele

Genital infections

Testicular disorders

Poor lifestyle

Nutritional deficiencies



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