Medical laboratory science
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🦠 GRAM POSITIVE COCCI β€” SHORT NOTES
πŸ”Ή Definition Gram positive cocci are spherical bacteria that retain crystal violet stain and appear purple under microscopy.
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πŸ”‘ Key Characteristics
βœ” Purple on Gram stain
βœ” Spherical shape (cocci)
βœ” Arranged in clusters, chains, or pairs
βœ” Non–spore forming
βœ” Some are normal flora, others pathogenic
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🦠 Important Gram Positive Cocci
1️⃣ Staphylococcus aureus
πŸ”Ή Arrangement: Clusters
πŸ”Ή Catalase: Positive
πŸ”Ή Coagulase: Positive
βœ… Diseases
β€’ Skin infections
β€’ Food poisoning
β€’ Osteomyelitis
β€’ Endocarditis
β€’ Toxic shock syndrome
πŸ§ͺ Lab Findings
β€’ Gram positive cocci in clusters
β€’ Golden yellow colonies
β€’ Beta hemolysis
🧫 Culture Media
β€’ Blood agar
β€’ Mannitol Salt Agar (MSA)
βš— Biochemical Tests β€’ Catalase positive
β€’ Coagulase positive
β€’ Mannitol fermentation positive
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2️⃣ Streptococcus pyogenes
πŸ”Ή Arrangement: Chains
πŸ”Ή Catalase: Negative
πŸ”Ή Group A Streptococcus
βœ… Diseases
β€’ Pharyngitis
β€’ Scarlet fever
β€’ Rheumatic fever
β€’ Cellulitis
πŸ§ͺ Lab Findings
β€’ Beta hemolysis on blood agar
β€’ Bacitracin sensitive
🧫 Culture Media
β€’ Blood agar
βš— Biochemical Tests β€’ Catalase negative
β€’ PYR positive
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3️⃣ Streptococcus pneumoniae
πŸ”Ή Arrangement: Diplococci
πŸ”Ή Alpha hemolytic
βœ… Diseases
β€’ Pneumonia
β€’ Meningitis
β€’ Otitis media
πŸ§ͺ Lab Findings
β€’ Lancet-shaped diplococci
β€’ Optochin sensitive
🧫 Culture Media
β€’ Blood agar
β€’ Chocolate agar
βš— Biochemical Tests
β€’ Bile soluble
β€’ Optochin sensitive
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4️⃣ Enterococcus faecalis
πŸ”Ή Normal intestinal flora
βœ… Diseases
β€’ UTI
β€’ Endocarditis
β€’ Wound infection
πŸ§ͺ Lab Findings
β€’Growth in 6.5% NaCl
🧫 Culture Media
β€’Blood agar
β€’ Bile Esculin agar
βš— Biochemical Tests
β€’ Bile esculin positive
β€’ PYR positive
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πŸ§ͺ Important Biochemical Tests Summary
βœ” Catalase Test
βœ” Coagulase Test
βœ” Optochin Sensitivity
βœ” Bacitracin Sensitivity
βœ” Bile Esculin Test
βœ” PYR Test
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Research methodology exam @Laboratorypractice
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Parasitology MCQ Series (Exam Focus)


πŸ“Œ Topic 1: Intestinal Flagellates
πŸ”Ή MCQs
1. Which parasite has a β€œface-like” appearance under microscope?
A. Entamoeba histolytica
B. Giardia lamblia
C. Trichomonas hominis
D. Balantidium coli
βœ… Answer: B. Giardia lamblia
2. Mode of transmission of Giardia lamblia is:
A. Vector bite
B. Skin penetration
C. Ingestion of cyst
D. Blood transfusion
βœ… Answer: C. Ingestion of cyst
3. Giardia infects mainly which part?
A. Colon
B. Small intestine (duodenum)
C. Liver
D. Blood
βœ… Answer: B. Small intestine
πŸ“Œ Topic 2: Amoeba
πŸ”Ή MCQs
4. Diagnostic feature of Entamoeba histolytica trophozoite:
A. Flagella
B. RBC ingestion
C. Cilia
D. Multiple nuclei
βœ… Answer: B. RBC ingestion
5. Infective stage of Entamoeba histolytica:
A. Trophozoite
B. Cyst
C. Sporozoite
D. Larva
βœ… Answer: B. Cyst
6. Characteristic lesion in amoebiasis:
A. Round ulcer
B. Flask-shaped ulcer
C. Linear ulcer
D. Nodular lesion
βœ… Answer: B. Flask-shaped ulcer

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Forwarded from Medical Lab Academy
Hematology Important point

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πŸ“š Short Notes | MCQs | Exam Focused Content

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