A laboratory technologist performs a manual microhematocrit and records a result of 42%. If the same sample were run on an automated electronic cell counter, the result would likely be slightly lower (approximately 39-41%). Why this would be?
Anonymous Quiz
31%
Red cell lysis due to high RPM
44%
Plasma trapping between packed red cells
20%
Inadequate mixing of the EDTA sample
5%
Evaporation of plasma during centrifugation
β€1
A 25-year-old male patient's lab results show a Hemoglobin (Hb) of 15 g/dl. According to the 'Rule of 3' used for quality control, what is the expected PCV range?
Anonymous Poll
61%
42% - 48%
18%
27% - 33%
18%
38.5% - 41.5%
4%
52% - 58%
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π§ͺ Basic Laboratory Rapid Test Procedures (For MLS Students & Practitioners)
π αα¬ α ααα α¨αααα α αα³αα΅ α¨Rapid Strip Tests procedures α₯αα¨αααα’
βββββββββββββββ
π΄ 1. HBsAg (Hepatitis B Surface Antigen) Strip Test Procedure
Sample: Serum / Plasma / Whole blood
Procedure:
Test kit α₯α sample αα° room temperature α αα‘
Test strip α¨pack α αα‘
2β3 drops sample α sample well αα α¨αα©
10β15 minutes αα α₯α
Result Interpretation: βοΈ 1 line (Control only) β Negative
βοΈ 2 lines (Control + Test) β Positive
β No Control line β Invalid
βββββββββββββββ
π΄ 2. Rheumatoid Factor (RF) Test (Latex Agglutination)
Sample: Serum
Procedure:
Test slide αα 1 drop serum α αα
1 drop RF latex reagent α¨αα©
Mixing stick α°α α αα α«αααα
Slide 2β3 minutes gently rotate α α΅αα
Result Interpretation: βοΈ Agglutination (clumping) β Positive
βοΈ No agglutination β Negative
βββββββββββββββ
π΄ 3. HIV Rapid Test (Strip Method)
Procedure:
Sample (blood/serum) αα¨αα©
Buffer α αα
15β20 minutes αα α₯α
Result: βοΈ 2 lines β Positive
βοΈ 1 control line β Negative
βββββββββββββββ
π΄ 4. Pregnancy Test (hCG Strip Test)
Sample: Urine
Procedure:
Strip α urine sample αα΅α₯ α«α΅αα‘ (2β3 sec)
Flat surface αα α«αα©
5 minutes αα α₯α
Result: βοΈ 2 lines β Pregnant
βοΈ 1 line β Not pregnant
βββββββββββββββ
β οΈ Important Notes: βοΈ Expiry date αααα α«α¨ααα‘
βοΈ Control line α«ααα£ β test invalid
βοΈ PPE (gloves, coat) αα αα
βοΈ Biohazard safety αα¨α°α
βββββββββββββββ
π For MLS Students:
α₯ααα tests ααα α’αα΅αα accuracy α proper procedure α₯α timing ααα α¨α³α!
π² Follow @Medical_lab_premium more MLS tutorials & practical guides
π αα¬ α ααα α¨αααα α αα³αα΅ α¨Rapid Strip Tests procedures α₯αα¨αααα’
βββββββββββββββ
π΄ 1. HBsAg (Hepatitis B Surface Antigen) Strip Test Procedure
Sample: Serum / Plasma / Whole blood
Procedure:
Test kit α₯α sample αα° room temperature α αα‘
Test strip α¨pack α αα‘
2β3 drops sample α sample well αα α¨αα©
10β15 minutes αα α₯α
Result Interpretation: βοΈ 1 line (Control only) β Negative
βοΈ 2 lines (Control + Test) β Positive
β No Control line β Invalid
βββββββββββββββ
π΄ 2. Rheumatoid Factor (RF) Test (Latex Agglutination)
Sample: Serum
Procedure:
Test slide αα 1 drop serum α αα
1 drop RF latex reagent α¨αα©
Mixing stick α°α α αα α«αααα
Slide 2β3 minutes gently rotate α α΅αα
Result Interpretation: βοΈ Agglutination (clumping) β Positive
βοΈ No agglutination β Negative
βββββββββββββββ
π΄ 3. HIV Rapid Test (Strip Method)
Procedure:
Sample (blood/serum) αα¨αα©
Buffer α αα
15β20 minutes αα α₯α
Result: βοΈ 2 lines β Positive
βοΈ 1 control line β Negative
βββββββββββββββ
π΄ 4. Pregnancy Test (hCG Strip Test)
Sample: Urine
Procedure:
Strip α urine sample αα΅α₯ α«α΅αα‘ (2β3 sec)
Flat surface αα α«αα©
5 minutes αα α₯α
Result: βοΈ 2 lines β Pregnant
βοΈ 1 line β Not pregnant
βββββββββββββββ
β οΈ Important Notes: βοΈ Expiry date αααα α«α¨ααα‘
βοΈ Control line α«ααα£ β test invalid
βοΈ PPE (gloves, coat) αα αα
βοΈ Biohazard safety αα¨α°α
βββββββββββββββ
π For MLS Students:
α₯ααα tests ααα α’αα΅αα accuracy α proper procedure α₯α timing ααα α¨α³α!
π² Follow @Medical_lab_premium more MLS tutorials & practical guides
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π§ͺ We Are Starting Laboratory Practice Today!
Hello dear members of @medical_lab_premium π©βπ¬π¨βπ¬
Starting from today, we are officially beginning practical laboratory sessions based on our schedule! This will help you gain real skills, not only theory
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Hello dear members of @medical_lab_premium π©βπ¬π¨βπ¬
Starting from today, we are officially beginning practical laboratory sessions based on our schedule! This will help you gain real skills, not only theory
Flsπ
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